A cost and time-to-completion analysis for all the methods used was done.

1: DNA extraction takes 10min + LAMP reaction and reading 50min. Capacity of hot-block is 24 samples: each determination comprises two tubes, one for P. falciparum and one for Plasmodium spp.+ controls (positive and negative for P. falciparum and P. vivax).2: To obtain the same sensitivity of examination as that for thick film at high power fields (with 100x oil immersion objective) for 10 min, a thin film should be examined for at least 30 min[13].3: Only treatment with ACT for patients with negative diagnoses with each method were considered.4: Treatment with ACT was considered exclusively.5: Total cost of diagnosis + total cost of treatment.A cost and time-to-completion analysis for all the methods used was done.