Shifts in functional groups of tree-associated fungi reveal decreasing tree health in German forest ecosystems

Data include the following zip files:Raw Illumina sequencing dataITS.rar (7.9 GB)including forward (R1) and reverse (R2) reads of each sample and replicate of the respective species and marker that have been sequenced. Reads were processed as described in the methods.OTU tablesBeech_ITS_clean.csv (975 KB)Spruce_ITS_clean.csv (1 MB)Trees_ITS.xlsx (5.7 MB)FungiTraits.xlsx (530 KB)including the OTU table of all species for the ITS marker, i.e. Fagus sylvatica and Picea abies. The OTU tables represent the "cleaned dataset" (see methods for details) after merging replicates and removing host DNA as well as any non-target DNA. Only OTUs with at least 177 bp in length and a match of >85% compared to the UNITE database are included. Separate OTU tables for Fagus sylvatica and Picea abies are further provided ("_cleaned"). A table with the respective fungal trait annoation is provided.R Codeincludes the R code for data filtering and data analysis.BuFi_benoit_filter_func_fulltime (37 KB)BuFi_benoit_filter_div_fulltime (115 KB)Supplementary TablesSupplementary tables containing additional information.Table S1: List of all samples including sampling location, year, respective speices, latitude and longitude of sampling locationTable S2: Details of the laboratory workflow