VC2.2.2 blocks binding of VEGF-C to VEGF-R2 and VEGF-R3 as measured by competitive ELISA.

2 nM biotinylated ΔNΔC-VEGF-C was preincubated with varying amounts of anti-VEGF-C scFv or control scFv and added on a VEGF-R2 or VEGF-R3 coated microtiter plate. Plotted datapoints are means from 4 replicates ± SEM. The datapoints were fitted to a sigmoidal dose-response curve model using GraphPad Prism 4. Inhibition of VEGF-C binding by anti-VEGF-C scFv reached significance vs the control scFv (*, p<0.05, Student's t-test) at molar excess of 100× more anti-VEGF-C scFv vs VEGF-C. #: p = 0.067 for anti-VEGF-C/VEGF-R2 vs control at molar excess of 33× more anti-VEGF-C than VEGF-C.