Impact of chronic extracellular acidosis on gene expression in mammary and pancreatic cancer cell lines

The study aim was to determine whether the microenvironmental acidosis in solid tumors contributes to cancer aggressiveness via changes in gene expression. RNA seq was carried out in parallel to a large array of phenotypical analyses of proliferation, growth, and invasiveness. Overall design: 3 human cancer cell lines (2 mammary, 1 pancreatic) adapted for 1-2 months to growth at extracellular pH 7.6 and 6.5, respectively, were subjected to RNA sequencing (BGI Hongkong), each in triplicates.