Human vascular smooth muscle cells undergoing cell senescence. Human vascular smooth muscle cells undergoing cell senescence

Vascular smooth muscle cells (VSMCs) undergo cell senescence in a number of diseases, both due to stress and loss of replication. Here we have cultured human VSMCs to replicative senescence, or induced stress-induced premature senescence (SIPS), and compared their transcriptome with cells treated with doxorubicin for 1d or control cells Overall design: Bulk RNAseq from primary human VSMCs undergoing (1) doxorubicin-induced SIPs (dox 1d+21d), (2) replicative senescence (RS), (3) Doxorubicin treatment for 1d (dox1d), or control cells (Ctr)