Effect of restricted dissolved oxygen on expression of Clostridium difficile toxin A subunit from E. coli.

We used microarray to identify the global gene expression profile of recombinant E.coli culture expressing repeating unit of the C. difficile toxin A (rARU) C-terminal region. Controlled condition batch runs were performed with E.coli expressing rARU at both restricted and unrestricted DO conditions for collecting samples of log and late-log phases at unrestricted and restricted DO. Time point mRNA profiling of E.coli expressing rARU at both restricted and unrestricted DO conditions for gene expression profiling.