Time-lapse microscopy of CAR-NKAE cells and memory CAR-T cells from multiple myeloma patients

Myeloma cells from U-266 cell line (green fluorescent cells) were seeded forming a monolayer on a six-channel polymer-treated flow chamber. Then a constant flux of either CAR-NKAE cells (red fluorescent cells, left) or memory CAR-T cells (red fluorescent cells, right) from the same MM patient was established over 20 min. Subsequently, four-hour observation of CAR-NKAE cell and memory CAR-T cell activity was performed.

U-266细胞系中的浆细胞瘤细胞（绿色荧光细胞）被接种于六通道聚合物处理过的流动室中，形成单层。随后，在20分钟内建立了来自同一浆细胞瘤患者的CAR-NKAE细胞（左侧红色荧光细胞）或记忆型CAR-T细胞（右侧红色荧光细胞）的恒定通量。随后，对CAR-NKAE细胞和记忆型CAR-T细胞的活性进行了四小时的观察。