RNA-seq on Epinephelus moara embryo under low temperature stress

Fish embryo cryopreservation is an important technique to preserve maternal and paternal gametes. Although extensive studies have focused on the development of cryopreservation technique in various kinds of fish, limited information was available on the effect of cooling and cryopreservation at the molecular level. In the present study, we utilized comparative transcriptome analysis among embryos cooled at different temperatures and embryos cultured in normal conditions. GO and KEGG enrichment were performed. Then, a quantitative PCR approach was enrolled to investigate the gene expressions of some differentially expressed genes following cooling at both 4 °C and -25.7 °C for up to 60 min. This study provided the availability of transcriptome dataset in cooled E. moara embryos for further exploration of the molecular mechanisms underlying fish embryo cryopreservation.