Supplementary Materials of article "Comparing DNA extraction methods for successful PacBio HiFi sequencing: a case study of the freshwater mussel Anodonta anatina (Bivalvia: Unionidae)" by Giulio et al.

This dataset contains the supplementary materials of the article "Comparing DNA extraction methods for successful PacBio HiFi sequencing: a case study of the freshwater mussel Anodonta anatina (Bivalvia: Unionidae)" by Giulio et al, published in the Peer Community Journal - section Genomics. BioRxiv preprint (reviewed at PCI Genomics) PCI Genomics recommendation by Abalde, 2026 Supplementary figure captions: Figure S1: Tapestation profiles of all DNA extractions, treatments and replicates from foot tissue of a single Anodonta anatina individual. Profiles are shown for both fresh and flash-frozen tissue across all extraction methods. Successful extractions were defined in the main text as those meeting thresholds for DNA yield (>1 µg total DNA), purity (A260/A280 ratio 1.8–2.0), and integrity (largest TapeStation peak >15 kb). Profiles with a dominant high-molecular-weight peak were considered more suitable for long-read sequencing, whereas smeared profiles or profiles dominated by low-molecular-weight fragments indicated reduced DNA integrity. Panels: A. Nanobind extractions. B. CTAB extractions. C. Omega extractions. D. MagAttract extractions. E.GenomicTip extractions. F. MegaLong extractions. G. Nanobind and CTAB extractions after DNA clean-up. Figure S2: TapeStation and Fragment Analyzer profiles of the 15 DNA extractions from foot tissue of a single Anodonta anatina individual that were submitted to the sequencing center in Lausanne, Switzerland. For each sample, the left panel shows the TapeStation profile generated at the extraction laboratory and the right panel shows the Fragment Analyzer profile generated at the sequencing center. These profiles were used to compare DNA integrity across instruments and to assess sample suitability for PacBio library preparation. Panels: A. CTAB extractions using fresh tissue. B. Nanobind extractions using fresh tissue. C. Omega extractions using frozen tissue. D. Nanobind extractions using frozen tissue. E. CTAB extractions using frozen tissue. Supplementary table captions: Table S1: Quality control results of all DNA extractions, treatments and replicates. Table S2: Quality control results of DNA extractions following DNA clean-up steps.