A single-cell transcriptomic atlas of fruitless-expressing neurons in the Drosophila pupal central nervous system

To examine the repertoires of genes expressed in individual fru P1-expressing neurons, we performed single cell sequencing. The analysis was performed on male and female central nervous system tissues (48-hour pupal stage), from flies that expressed membrane-bound GFP in fru P1 neurons. We chose this developmental stage to gain further insight into how genes direct development of fru P1 neurons, as this is the stage where FruM has peak expression (in ~2,000 neurons). Overall design: scRNA-seq using 10x Genomics CHROMIUM Single Cell 3' Solution V3 Chemistry of 48hr after puparium formation (APF) fru P1-expressing cells from the male and female Drosophila Central Nervous System (CNS).