Transcriptomic respponses in the gut in Daphnia magna across toxic chemicals that inhibit feeding and food limiting conditions

The aim of this study was to assess the transcriptomic responses in guts dissected from Daphnia magna individuals exposed to concentrations of selected compounds that peroduced low and high inhibitory effects on feeding. Chemical treatments included Cd, Cu, fluoranthene, l-cyalothrine and the cyanotoxin anatoxin a. Two additional treatments including low and no food (starving) were also included to better account for transcriptomic responses related to food limitation. We tested the hypothesis that the studied chemicals should share similar molecular signalling pathways across low and high levels of feeding impairment. Daphnia magna was exposed to Cd, Cu, fluoranthene, cyalothrin and anatoxin a at 2 feeding inhibition levels. 3 different food levels and controls were also added to the experiment. 5 replicates per treatment with 3 random samples used to hybridization. The extracted guts were RNA extracted and this material used for microarray hibridization