Comparision of LPS/GalN-induced gene expression in Rack1F/F and Rack1F/F;Alb-cre livers.

In order to further discover the resistance mechanism of Rack1F/F;Alb-cre mice to LPS/ GalN-induced fulfulant hepatitis, we used genome-wide microarray expression profiling as a discovery platform to identify potential genes associated with resistance to LPS/ GalN-induced in Rack1F/F;Alb-cre mice. Fulminant hepatitis was induced by LPS/GalN in Rack1F/F;Alb-cre mice and Rack1F/F mice for 0, 1,3 and 6 hours, respectively. LPS/GalN induced gene expression in Rack1F/F;Alb-cre mice and Rack1F/F mice were measured at 0,1,3,6 hours after exposure to LPS (35ug/kg) and GalN (1000mg/kg). Two independent experiments were performed at each time point and each used different donors for each experiment.