Prp4-1 temperature shift

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Prp4-1 and wt strains were grown at 26_C to A600 of 1.0, then an equal volume of 48_C media was added to bring the temperature to 37_C

将Prp4-1菌株与野生型（wild type，缩写wt）菌株在26摄氏度条件下培养至A600值为1.0，随后加入等体积的48摄氏度培养基，将培养体系的温度调整至37摄氏度。