Quantitative analysis of protein expression in PeCa.

The expression of fluorophore labelled proteins (CD1, Cy3; MMP7, FITC; c-MYC, Cy5; Wnt4 Alexa Fluor-405, see Fig 1) was quantified in an unbiased manner, by using a reproducible, semi-automated particle analysis protocol (see methods) using grayscale images of the stained tissue from 141 tissue cores (102 malignant and 37 non-malignant control, normal or cancer adjacent penile tissue cores). Data is presented as means ± SD for control vs malignant samples (all grades). Fold increase is relative to control for each protein and significance of difference was calculated using Mann Whitney U test.Quantitative analysis of protein expression in PeCa.