Long non-coding RNAs from divergent transcription of protein-coding genes. Mus musculus

A remarkable number of long non-coding RNA (lncRNA) species have been identified in mammalian cells, but the genomic origins of these molecules in individual cell types is poorly understood. As a prerequisite to studying the transcriptional regulation of lncRNAs, we conducted a comprehensive analysis of the genomic origins of lncRNAs expressed in embryonic stem cells (ESCs). Overall design: Polyadenylated RNA and total RNA depleted of ribosomal content was used for preparation of two independent sequencing libraries