TimeLapse-seq reveals that ALKBH5 modulates RNA stability of metabolic transcripts

N6-methyadenosine (m6A) RNA modification controls numerous cellular processes through regulation of RNA stability and translational efficiency. Whether the RNA m6A modification regulates energy metabolism process by posttranscriptional regulation is unknown. We here show that loss of the m6A demethylase ALKBH5 results in instability of oxogluatarate-dehydrogenase (Ogdh) messenger RNA and transcripts of other metabolic enzymes. Overall design: RNA isolated from wildtype and Alkbh5-deficient murine lineage-depleted bone marrow cells treated +/- s4U was subjected to oxidative-nucleophilic-aromatic-substitution chemistry. Sequencing libraries were prepared from all RNA.