Assessment of the presence or absence of the 627K and 701N mutations in the PB2 segment of viruses recovered from the nasal washes of H9N2 influenza virus infected or exposed ferrets.

aResults were obtained by using a deep sequencing approach.
bWe extracted viral RNA from nasal washes collected from virus-inoculated and virus-exposed ferrets. PB2 and HA were amplified and cloned into T vectors. Ten molecular clones from each sample were randomly selected and sequenced. No changes in HA were detected. For values in parentheses, the number on the left of the slash shows the number of clones bearing the indicated amino acid at position 627 or 701, and the number on the right of the slash shows the total number of clones sequenced. ND, not done; /, not applicable.
Assessment of the presence or absence of the 627K and 701N mutations in the PB2 segment of viruses recovered from the nasal washes of H9N2 influenza virus infected or exposed ferrets.