METAGENOMIC ANALYSIS OF RESHI HOT SPRING SIKKIM INDIA

The thermophilic bacteria are recognized by their metabolic thermo-stability which are buoyed by their thermophilic protein. The thermostability of the thermophilic enzymes has been established as valuable biocatalysts for various biotechnological and industrial purposes. A prototype is Taq-polymerase from Thermus aquaticus that led to the advance of the polymerase chain reaction (PCR) technique . To understand the Geochemistry, Geomicrobiology, bioenergetics and biotechnological potential of geothermal systems, various studies have been performed worldwide. However, the conventional classical culture-dependent approach was the primary and sole technique to determine the Geomicrobiology of the hot springs before the development of neo molecular techniques. Inability to culture the vast majority of microorganisms with the culture-dependent method has questioned the technique for inclusive profiling of different environment. To overcome this limitation, different culture-independent techniques including DGGE, PLFA, and Metagenomic studies have revealed a subsequent increase in microbial molecular ecology studies.