Additional file 1: of Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platform
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Table S1. PCR primer sequences for 8 genes. Table S2. Mis-assignment rate of control group with Q30 filter. Table S3. Total contamination rates for each pooling scenario in Fig. 3a. Table S4. Total reads and rates of all WGS libraries (indexes 1â8). Table S5. Percentage of DNA sharing the same barcode with neighbours. Table S6. Effect of Q30 filter on sequencing reads and rates when library pooling is performed after PCR amplification (indexes 1â8). Table S7. Index contamination in water control with PCR-free library. Table S8. Raw data of PCR-free library contamination, 3 lanes. (XLSX 138 kb)
创建时间:
2019-03-14



