Table S4.pdf

Table S4. N₂O production by Methylocystis sp. strain SC2 in response to increasing NH₄⁺ concentrations. Note: SC2 cells were incubated in 120-ml serum bottles under a headspace CH₄:air mixing ratio of 20:80 (v/v) at 25 ℃, with shaking at 130 rpm. Using an N₂O microsensor with piercing-needle (Unisens A/S, Denmark), the production of N₂O in the headspace was regularly monitored during the whole incubation time period (14 days). Significant difference between comparison groups was calculated using one-way ANOVA Bonferroni t-test: *<em>p</em>-value≤0.05, **<em>p</em>-value≤0.01, ***<em>p</em>-value≤0.001 [comparing the same incubation time point across the increasing NH₄⁺ concentrations (10 mM, 30 mM, 50 mM, 75 mM NH₄⁺) relative to the control treatment (1 mM NH₄⁺)]; and ^ǂ<em>p</em>-value≤0.05, ^ǂǂ<em>p</em>-value≤0.01, ^ǂǂǂ<em>p</em>-value≤0.001 [comparing the same NH₄⁺ treatment over incubation time relative to the early_log phase]. See Fig. 5 for the graphical presentation of N₂O production over the whole incubation period.