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Supplementary Tables: A qPCR-based method for rapid quantification of six intestinal homeostasis relevant bacterial genera in feces

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DataCite Commons2024-05-17 更新2025-04-15 收录
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https://tandf.figshare.com/articles/dataset/Supplementary_Tables_A_qPCR-based_method_for_rapid_quantification_of_six_intestinal_homeostasis_relevant_bacterial_genera_in_feces/15097347
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<b>Supplementary Table 1</b>. <b>Bacterial species and strains used for six genus-specific primer pairs design<sup>a</sup></b> <sup>a</sup>16S rRNA sequences of above species or strains covering <i>Bacteroides</i>, <i>Lactobacillus</i>, <i>Pediococcus</i>,<i> Enterococcus</i>, <i>Streptococcus</i>, <i>Bifidobacterium, Prevotella</i>, <i>Clostridium</i>, <i>Eubacterium, Ruminococcus,</i><i> Leuconostoc</i>, <i>E. coli</i> and <i>Klebsiella pneumoniae</i> were retrieved from the NCBI GenBank database, and aligned to determine the regions designed for primers. The bacteria in the same box were closely related. <b> </b> <b>Supplementary Table 2.</b> <b>Level of similarity for 16S rRNA gene of 13 bacterial genera mentioned in Table S1.</b> <b> </b> 16S rRNA gene sequence of 13 different bacterial genera used in Table S1 were aligned with each other, respectively. The similarity levels were detected by DNAMAN8.0. <b> </b> <b>Supplementary Table 3. </b> <b>Comparison between available primers and novel primers designed for six bacterial genera in this study.</b>
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Taylor & Francis
创建时间:
2021-08-03
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