Effect of pm2.5 maternal exposure on DNA methylation in the offspring myocardium

The animals were divided into two groups according to exposure of PM2.5 (particulate matter less than 2.5 µm): control group (Non-PM group) or PM exposure group (PM group).PM2.5 at 150 μg/m3were administered by intratracheal instillation during the gestation period of female rats from Day 1 of conception onwards.After the female rat gave birth,the offspring's heart DNA was extracted and anlyzed. DNA promotor methylation profile were determined.The goal was to determine the effects of PM2.5 exposure on the DNA promotor methylation patterns in heart tissue.