IgSF11 cooperates with RAP1 to promote cell migration and invasion of cutaneous melanoma

IgSF11 is a member of the immunoglobulin superfamily and is homologous to the Junction Adhesion Molecule (JAM) family. IgSF11 is expressed exclusively in the testis and central nervous system in normal adult tissues. On the other hand, ectopic expression of IgSF11 at the mRNA level has been detected in several malignant tumors, including malignant melanoma. However, the lack of highly specific antibodies has prevented functional analysis of IgSF11 in cancer. In this study, we developed a specific monoclonal antibody against IgSF11 and evaluated the IgSF11 expression of malignant melanoma cases at the protein level by immunohistochemistry. As a result, more than half of the cases showed high expression of IgSF11. In this presentation, we will show the clinical significance of IgSF11 and its molecular function in malignant melanoma cell lines. Overall design: RNA-seq analysis was performed using wild-type and IgSF11-over expression or IgSF11-knockout melanoma cell lines (A375 and 888mel).