Nanopore RNA sequencing in S. cerevisiae strains defective for decapping and deadenylation

RNA turnover in eukaryotes is triggered by decapping, believed to be activated mostly after deadenylation. To investigate simultaneous changes in RNA levels and poly(A) tail size we performed Nanopore RNA sequencing on total RNA extracted from S. cerevisiae strains in which the decapping enzyme Dcp2, or the deadenylation factors Ccr4 and Pop2 were depleted through an inducible degron system. Two fully independent replicate experiments were performed for wild type and the two mutant conditions.真核生物的RNA周转（RNA turnover）由脱帽反应（decapping）所触发，学界普遍认为脱帽反应主要在脱腺苷酸化（deadenylation）之后被激活。为探究RNA水平与多聚(A)尾长度的同步变化，我们对通过诱导型降解子系统（inducible degron system）实现脱帽酶Dcp2或脱腺苷酸化因子Ccr4、Pop2功能耗尽的酿酒酵母（S. cerevisiae）菌株所提取的总RNA，开展了纳米孔RNA测序（Nanopore RNA Sequencing）。针对野生型菌株与上述两种突变株条件，我们完成了两组完全独立的重复实验。