Single cell RNA-seq of 3 key time points in oesophageal post-natal development

The purpose of this single cell experiment is to compare and characterize at molecular level oesophageal epithelial cells at different points in post-natal development (post-natal day 7 and 28) and at the onset of homeostasis (over p70). With the aim of identifying differences in timepoints showing the key molecular markers of the transition from a rapidly expanding tissue to one that is stable in size and function. Previous data has suggested this transition point occurs around P28 and we hope to see here a similarity in the profile of P28 samples with that of adults. Single cell suspensions of pooled oesophageal epithelial and stroma cells were sorted for EpCam+ and CD45- expression to select for epithelium cells for RNA-sequencing.