Figure 1C_Heat Map of gH/gL binding screen
收藏Figshare2022-04-13 更新2026-04-08 收录
下载链接：
https://figshare.com/articles/dataset/Figure_1C_Heat_Map_of_gH_gL_binding_screen/19141997/1
下载链接
链接失效反馈官方服务：
资源简介：
Mice were repeatedly immunized with HCMV purified virus or plasmid encoding gH/gL and blood was collected to evaluate the neutralization capacity of mouse sera in epithelial (ARPE-19) and fibroblast (MRC5) cells using reporter virus AD169R (MOI 0.1). Percent infection was quantified using GFP and normalized to virus incubated in the presence of normal mouse serum (NMS). Each serum condition was tested in technical triplicate (n=3). (c) Antibody clones capable of binding gH/gL were identified following hybridoma supernatant incubation with 293ExpiF cells transiently expressing gH/gL. Mean fluorescence intensity (MFI) was determined for each sample and summarized by fusion (n=1).
提供机构：
Tortorella, Domenico; Parsons, Andrea
创建时间：
2022-02-10
