RNA-seq of Staphylococcus aureus isolates from chronic rhinosinusitis patients (CSS) against healthy individuals

S. aureus isolates from chronic rhinosinusitis patients and healthy individuals were cultured overnight. The strains belonged to clonal complexes CC7 (CSS66 and MIN142) and CC15 (CSS126 and MIN93) that are both prevalent in Germany. These isolates have previously been characterized in Tuchscherr, L., et al., Reduced Glycolysis and Cytotoxicity in Staphylococcus aureus Isolates from Chronic Rhinosinusitis as Strategies for Host Adaptation. Int J Mol Sci, 2024. 25(4). Cultures were diluted in TSB broth to an optical density (OD600) of 0.05 the following day, and samples were collected at 3- and 6-hours post-dilution. Total RNA was extracted at these time-points and subjected to RNA sequencing (RNA-seq).