Analysis of proteins domains of Src involved in Pyk2 phosphorylation.

A, SYF cells transiently transfected with Src-WT or the various Src mutant constructs were serum-starved, detached and kept in suspension in DMEM containing 0.5% BSA for 1 hr. Cells were then seeded on fibronectin-coated dishes, and allowed to attach for 30 min. Phosphorylation of Pyk2 and FAK was examined by immunoblot. B, Co-IP of Src and Pyk2. SYF cells were transiently transfected with Myc-Pyk2 along with Src-WT or Src mutant constructs, the expressed Src was immunoprecipitated and the associated Pyk2 was examined by immunoblot.