S1 File - A novel yeast-derived aldehyde-reducing compound MF001 protects against alcohol-induced liver damage

S1 Fig. MF001 reduces fatty acid oxidation and EtOH regulating genes. (A) Fatty acid oxidation gene expression levels of Pparα, Pgc-1α, Cpt-1α, and Cpt-1β determined using qPCR in EtOH-, PA-, and MF001-treated mouse primary hepatocytes. (B) Ethanol reducing gene expression levels of Cyp2e1, Adh1, and Aldh2 in determined using qPCR in EtOH-, PA-, and MF001-treated mouse primary hepatocytes. (C) Enzyme activity levels of ALDH2 in primary hepatocytes determined by ELISA. Values represent the mean ± SEM. *p **p ***p #p ##p ###p S2 Fig. MF001 reduces fatty acid oxidation and protects against alcohol-induced liver inflammation. (A) Expression of major fatty acid oxidation genes Pparα, Pgc-1α, Cpt-1α, and Cpt-1β determined using qPCR in mouse liver tissues. (B, C) Activity of inflammatory enzymes TNF-α and IL-1β and ethanol reducing enzyme ALDH2, determined using serum mouse samples by ELISA. Values represent the mean ± SEM. *p **p #p ##p S3 Fig. MF001 attenuates alcohol-induced fatty acid oxidation and relieves alcohol-induced inflammation. (A) Expression of major fatty acid oxidation genes Pparα, Pgc-1α, Cpt-1α, and Cpt-1β alcohol induced and MF001 treated mouse liver tissues, determined using qPCR. (B, C) Activity of inflammatory enzymes TNF-α and IL-1β and ethanol reducing enzyme ALDH2, in serum of alcohol induced and MF001 treated mouse samples by ELISA. Values represent the mean ± SEM. *p **p ***p #p ##p S4 Fig. Graphical Abstract. Schematic representation for the function of MF001 in liver. S1 Table. Primer sequences used for qPCR. S1 Data. Western blot raw image. S2 Data. Statistical analysis for One-way ANOVA. (ZIP)