Next Generation Sequencing Facilitates Quantitative Analysis of Normal human keratinocytes and IL-13 receptor alpha 1 and 2 knockdown human keratinocytes Transcriptomes

Purpose: The goals of this study are to investigate the influence of human keratinocyte IL-13 receptor knockdown on transcriptome profiling (RNA-seq) Methods: A total of 18 samples were tested using the DNBSEQ platform, with an average yield of 1.13G data per sample. The average alignment ratio of the sample comparison genome was 95.24%, which was comparative The average alignment of the gene set was 83.86%; a total of 19022 genes were detected. Conclusions: Our study represents the first detailed analysis of transcriptoms after human keratinocyte with IL-13 receptor alpha 1 and 2 knockdown transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. We conclude that numerous gene transcription were affected after knockdown of each IL-13 receptor. Human keratinocyte mRNA profiles after knockdown of IL-13 receptor alpha 1 and 2