Comparing the proteome of human induced pluripotent stem cells cultured during normoxic versus hypoxic conditions

Human induced pluripotent stem cells (hiPSCs) have become an invaluable tool to study molecular disease mechanisms on a human genetic background as they can be differentiated in different cell types including cardiac myocytes. One major downside that has decelerated the research performed in hiPSCs is that during propagation of these cells, changes in the karyotype of these cells have been observed. Interestingly, when hiPSCs are being cultured under hypoxic conditions and not as commonly done under normoxic conditions, hiPSCs grow faster to confluency and also changes in the karyotype of these cells are less frequently observed.