The role of RUNX2 and BHLHE40 in pathologically relevant CD4-positive tissue-resident T-cells in Crohn's disease. (CITE-seq, CD4+ T cell)

Tissue-resident T-cells (TRM) are deeply involved in immune memory at the site of inflammation. Here, we identified two key transcription factors, RUNX2 and BHLHE40 as regulators of pathologically relevant CD4-positive TRM in the inflamed gut mucosa of Crohn’s disease patients. CD4+ T cells were sorted from mononuclear cells derived from surgically resected fresh gut specimens by flow cytometry. Each specimen was labeled with HTO antibodies and subjected to single-cell CITE-seq analysis details of the HTO antibodies: Totalseq-C Human Universal Cocktail (supplementary file: TotalSeq_C_Human_Universal_Cocktail_v1_137_Antibodies_Barcodes.xlsx, BioLegend) was used.