Differential gene expression analysis between Wild type(WT) and pbl19/pPBL19C3A-GFP-HA transgenic plants

Purpose: The goals of this study is compared the differential gene expression between WT and pbl19/pPBL19C3A-GFP-HA transgenic plants Methods: For RNA-seq analyses, a total amount of 1 mg RNA per sample was used for RNA-seq library construction. RNA-seq on the BGISAQ-500 with 150 bp paired-end reads and data analysis were carried out by the Beijing Genomics institution Results: RNA-seq analysis of Col-0 and pbl19/pPBL19::PBL19C3A at 28DAG.Numbers of differentially expressed genes in pbl19/pPBL19::PBL19C3A plants compared to WT.1757 differentially expressed genes were constantly up-regulated in pbl19/pPBL19::PBL19C3A plants compared to WT Conclusions: Receptor-like cytoplasmic kinase PBL19 exhibited the highest transcriptional upregulation among the forty-six genes of the RLCK VII subfamily. 699 differentially expressed genes was down-regulated in pbl19/pPBL19::PBL19C3A plants compared to WT. Overall design: mRNA of 28-day old wild type (WT) and pbl19/pPBL19C3A-GFP-HA transgenic plants