Homo sapiens Transcriptome or Gene expression. Homo sapiens

The overexpression plasmids of lincRNA-ASAO and the negative control group were designed and constructed by OBiO Technology (Shanghai, China). The vector components used for overexpressing lincRNA-ASAO included pASLenti-pA-MCS-CMV-EF1-mCherry-P2A-BSR-WPRE. According to the manufacturer's instructions, hDPSCs at passages 3-5 were seeded in cell culture flasks and transfected with the corresponding lentiviral vectors to generate stable cell lines for subsequent experiments. The transfection efficiency was validated by quantitative real-time quantitative PCR (qRT-PCR) and western blotting. hDPSCs were transfected with lentivirus and divided into LV-NC group and LV-lincRNA-ASAO group. hDPSCs were prepared to 1.0*10^5 cells/ml in PBS. After inducing stable hDPSC cell lines overexpressing lincRNA-ASAO and their negative control for 14 days toward odontoblast-like cells, total transcriptome RNA was collected and sent to Ribobio Company (Guangzhou, China) for sequencing. The overexpression plasmids of Polypyrimidine Tract Binding Protein 1 (PTBP1 or hnRNPI) and the negative control group were designed and constructed by OBiO Technology (Shanghai, China). The vector components used for overexpressing PTBP1 included pSLenti-SFH-EGFP-P2A-Puro-CMV-3xFLAG-WPRE. The transfection and validation methods were the same as above. hDPSCs were transfected with lentivirus and divided into LV-NC group and LV-PTBP1 group. hDPSCs were prepared to 1.0*10^5 cells/ml in PBS. After inducing stable hDPSC cell lines overexpressing PTBP1 and their negative control for 14 days toward odontoblast-like cells, total transcriptome RNA was collected and sent to Ribobio Company (Guangzhou, China) for sequencing.