dab-1 (DAB (Drosophila disabled) homolog)

dab-1 encodes an ortholog of the cytoplasmic adaptor protein DISABLED, required for normal molting and meiotic arrest; DAB-1 is also required for EGL-17 secretion from vulval cells, and thus indirectly for normal sex myoblast (SM) migration and egg-laying; DAB-1 contains a conserved PTB domain and signals that confer localization to Golgi-proximal vesicles, and DAB-1 localizes to vesicular structures in vivo; dab-1 is expressed in ventral precursor cells, and DAB-1 prevents EGL-17 protein accumulation in them; dab-1 is also expressed in other cells (anchor cell, sheath cells, etc.) but has no obvious function in them; DAB-1 binds the cytoplasmic domains of either LRP-1 or LRP-2 in yeast two-hybrid experiments; dab-1(gk291) and dab-1(RNAi) animals have similar defects in molting, EGL-17 secretion from VPCs, SM migration, and egg-laying; dab-1(RNAi) does not enhance the SM phenotypes of lrp-1(RNAi) or lrp-2(RNAi), suggesting that these genes act in concert to promote normal EGL-17 secretion and SM migration; in vitro, DAB-1 binds to the ear domains of APT-4 (strongly) and of APT-1 and APT-9 (weakly); genetically, dab-1(RNAi) functions as part of the vab-1 pathway in oocytes inhibiting meiotic maturation, and DAB-1 is found in oocyte cytoplasm.

dab-1基因编码细胞质衔接蛋白DISABLED（DISABLED）的同源物，其对于正常蜕皮与减数分裂停滞是必需的；DAB-1蛋白同时是阴门细胞分泌EGL-17蛋白所不可或缺的，进而间接参与调控正常的性成肌细胞（sex myoblast，SM）迁移与产卵过程；DAB-1包含保守的PTB结构域（PTB domain），以及介导其定位于高尔基体近端囊泡的信号序列，且在体内定位于囊泡结构；dab-1基因在腹侧前体细胞中表达，且DAB-1蛋白可抑制该类细胞内EGL-17蛋白的积累；dab-1基因还在其他细胞（如锚定细胞、鞘细胞等）中表达，但在这些细胞中未观察到明显的生物学功能；在酵母双杂交实验中，DAB-1蛋白可结合LRP-1或LRP-2的胞质结构域；dab-1(gk291)突变体与dab-1(RNA干扰（RNAi）)个体在蜕皮、阴门前体细胞（VPCs）分泌EGL-17蛋白、SM迁移以及产卵方面呈现相似的缺陷表型；dab-1(RNAi)不会增强lrp-1(RNAi)或lrp-2(RNAi)的SM表型，提示上述基因协同发挥作用以促进正常的EGL-17分泌与SM迁移；体外实验显示，DAB-1蛋白可强力结合APT-4的Ear结构域，对APT-1与APT-9的Ear结构域结合活性较弱；遗传学实验表明，dab-1(RNAi)作为卵母细胞中抑制减数分裂成熟的vab-1信号通路（vab-1 pathway）的组分发挥功能，且DAB-1蛋白定位于卵母细胞胞质中。

搜集汇总

背景与挑战

背景概述

该数据集总结了dab-1基因的功能和特点，它编码DISABLED蛋白的同源物，在蜕皮、减数分裂停滞和EGL-17分泌中起关键作用，并参与性成肌细胞迁移；dab-1包含PTB结构域，定位于囊泡结构，与LRP-1/LRP-2和AP复合物亚基相互作用，属于vab-1通路调控卵母细胞成熟。