bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis [RNA-seq]

To understand the function of AtbZIP17 in heat stress responses at reproductive stage, we performed RNA-seq analysis of heat-stressed flowers (stage 1-12) in WT and bzip17 mutant (SALK_104326) plants with 38°C for 6 hours, and the control plants were kept at 22°C. There were 12 samples in RNA-seq analysis, wild-type and bzip17 mutant plants at flowering stage were treated at 22°C and 38°C for 6 hours. The unopened flower buds (stage 1-12) from 8 plants were collected and immediately frozen in liquid nitrogen with a total of 3 biological replicates for each treatment. The analysis was processed in according to standard protocols on Illumina Novaseq™ 6000.