Expression data from mouse neonatal hindlimb muscles

During neonatal development, skeletal muscle grows dramatically by myonuclei accretion to existing fibers and hypertophic growth of fibers with protein synthesis. To understand molecular mechanism underlying neonatal muscle growth, we used microarray to profile the global program of gene expressions especially involved in myoblast fusion, migration, and muscle fiber growth by itself. Mouse hindlimb muscles at postnatal day (P) 2, 7, and 14 were isolated and used for RNA extraction and hybridyzation on microarrays. We used three biological replicates for each time point.在新生发育阶段，骨骼肌主要通过两种方式实现显著生长：一是肌核向现有肌纤维的募集附着，二是伴随蛋白质合成的肌纤维肥大性生长。为阐明调控新生期骨骼肌生长的分子机制，本研究采用基因芯片（microarray）技术，对参与成肌细胞融合、细胞迁移以及肌纤维自主生长的全局基因表达程序进行了分析。本研究采集了出生后第2、7、14天（P2、P7、P14）的小鼠后肢肌肉组织，用于RNA提取及基因芯片杂交实验。每个时间点均设置3次生物学重复。