DNA sequencing at picogram level to investigate life on Mars and Earth

For studying life within the rocks of Earth and Mars, we demonstrate, in an ISO 5 clean room, a procedure based on MinION nanopore technology that correctly identifies organisms with only 2 pg of DNA without amplification, which represents unprecedented sensitivity. The folder contains fast5 and fastq passed and failed sequencing files of all the experiments conducted to investigate ONT's MinION ability to detect and characterize extremely low biomass.