WGS of DNA lesions from irradiated and non-irradiated HaCaT cells

This study aims to investigate the binding DNA lesions of cGAS in HaCaT cells exposed to 0Gy and 20Gy by WGS. In this study, HaCaT cells were maintained in DMEM. All culture media were supplemented with 10% fetal bovine serum (FBS;Biological Industries, Kibbutz Beit-Haemek, Israel). Cells were grown at 37 degree Celsius in 5% CO2 incubators. Cells were exposed to 20Gy of ionizing radiation using X-ray linear accelerator (Rad Source, Suwanee, GA) at a fixed dose rate of 1.15 Gy/min.Two hours after irradiation, specific antibody against cGAS was used to pull down cGAS and the binding DNA lesions, which was then analyzed through WGS.