National Key Research and Development Program of China

MccJ25 displays significant antibacterial activity, a well-defined mode of action, exceptional safety, and remarkable stability. Hence, it presents itself as a compelling candidate for an optimal antibacterial or anti-endotoxin medication. The successful establishment of exogenous production of MccJ25 in B. subtilis provides a strategy for reducing its production cost and diversifying its utilization. This project developed several better engineering strains of B. subtilis, enhancing the production of MccJ25 and elucidating the factors influencing its regulatory expression and accumulation.