Identifying functional enhancers regulating Cebpa expression and adipocyte differentiation (mRNA-Seq)

To explore the effect of enhancer repression on adipocyte differentiation in a transcriptome-wide manner, we infected cells with 1 mL lentiviruses medium containing dCas9-KRAB-En2-sgRNAs (Experimental group) or dCas9-KRAB (Control group) at the multiplicity of infection (MOI) of 100 for 12 h. We performed transcriptional profiling by RNA sequencing (RNA-seq) for dCas9-KRAB-En2 and dCas9-KRAB cells at day 7 of differentiation. Overall design: Gene expression profiling analysis of mRNA-seq data for dCas9-KRAB cells and dCas9-KRAB-En2 cells on days 7 of differentiation.