Milk-clotting properties on bovine caseins of a novel cysteine peptidase from germinated Moringa oleifera seeds

Electrophoresis of α-CN (A), β-CN (B), and κ-CN (C) hydrolyzed by calf rennet. Lane M, left side: the three casein fractions hydrolyzed separately by calf rennet in a 1:10 (enzyme/substrate; v/v) ratio for 5 min and 3 h; Lane M (Fig. D), right side: whole casein and the three casein fractions hydrolyzed separately by calf rennet in a 1:1 (enzyme/substrate; v/v) ratio for 1 h.