MOESM3 of Blood-derived dendritic cell vaccinations induce immune responses that correlate with clinical outcome in patients with chemo-naive castration-resistant prostate cancer

Additional file 3: Figure S3. Myeloid and plasmacytoid vaccine characteristics. (A) Purity of freshly isolated pDC and mDC was analyzed by flow cytometry and based on expression of CD123 and BDCA2 (pDC) or CD1c with absence of CD20 (mDC). (B) Yield of pDCs and mDCs after isolation with CliniMACS Prodigy. (C) Phenotype, (D) viability and (E) cytokine production of pDC and mDC after maturation with protamine-mRNA complexes. Phenotype was analyzed by flow cytometry. Cytokine production was analyzed in the supernatant by cytometric bead array.