Alphafold2 and AlphaFold-Multimer Predicted Interactions of Soybean Proteins with Macrophomina phaseolina Effectors reveals putative protease inhibitors and SUSS effectors.

Kunitz Monomer Prediction:

Data: Analysis of soybean Kunitz proteins.

Details: Detected on the apoplast at 3 days post-infection with Macrophomina phaseolina.

File: KUNITZ_monomers_outputdir.zip

Uncharacterized M. phaseolina Protein Monomer Prediction:

Data: Predictions for uncharacterized proteins.

Details: Detected on the apoplast at 3 days post-infection.

File: uncharacterised_proteins_SUSS_effectoroutputdir.zip

Positive Validation Set:

Data: Experimental verification of protein-inhibitor pairs.

Details: Pairs include experimentally verified interactions, specifically proteins and inhibitors, but lack resolved crystal structures.

File: existing_non_existinpairs_Validation_outputdir.zip

Soybean Serine Protease-Kunitz Interaction:

Data: Interactions between soybean serine proteases and Kunitz proteins.

Details: Analyzed in the apoplastic space at 3 days post-infection.

File: glycine max_Serine protease_Vs_Gmaxkunitz_outputdir.zip

Cysteine Protease without Pro-domain-MoErs-like effector Interaction:

Data: Interactions involving cysteine proteases.

Details: Rice RD21 and soybean cysteine proteases with pro-domains removed interacting with MoErs1 and MoErs1-like M.phaseolina effectors.

File: AF2-Multimer_RD21&GmaxCproteases_MoERS1_screening_outputdir.zip

Fungal Serine Protease-Kunitz Interaction:

Data: Interactions between Macrophomina phaseolina serine proteases and soybean Kunitz proteins.

Details: Evaluated in the apoplastic space at 3 days post-infection.

File: fungalSerineprotease_Vs_Gmax_kunitzoutputdir.zip

Negative Validation Set:

Data: Known non-interacting pairs.

Details: Non-interacting pairs of serine proteases-chitinases that are not resolved as crystal structures

File: Gmax_Serineprotease_Vs_Gmaxchitinases_Validation_outputdir.zip