Sequencing results of the gut microbiota in zebrafish after the use of anti-Vibrio vulnificus immunoglobulin Y (IgY) antibodies

This study investigates the protective effects of immunoglobulin Y (IgY) antibodies as a targeted treatment against Vibrio vulnificus infection in zebrafish (Danio rerio), focusing on survival outcomes and microbiota modulation. Zebrafish were administered IgY antibodies through injection and oral routes prior to V. vulnificus exposure. Results showed that IgY treatment significantly enhanced survival rates, with injected groups achieving up to 90% survival compared to only 40% in untreated controls. This protective effect is attributed to IgY pathogen-specific action against V. vulnificus, limiting bacterial load and infection severity. Additionally, 16S rRNA sequencing revealed favorable shifts in gut microbiota among IgY-treated fish, with increased abundances of beneficial bacteria such as Lactobacillus and Faecalibacterium prausnitzii, alongside reduced levels of pathogenic genera like Vibrio and Aeromonas. These findings highlight IgY dual benefits: pathogen-specific inhibition an..., , , # Sequencing results of the gut microbiota in zebrafish after the use of anti-Vibrio vulnificus immunoglobulin Y (IgY) antibodies

[https://doi.org/10.5061/dryad.q2bvq83w0](https://doi.org/10.5061/dryad.q2bvq83w0)

## Description of the data and file structure

Bacteria_A1_raw.fq.gz、Bacteria_A2_raw.fq.gz、Bacteria_A3_raw.fq.gz :

Model groups (A1, A2, A3 Three replicates.): Adult zebrafish weighing 0.2 g were injected with 10 μL of Vibrio vulnificus resuspended in PBS solution (approximately 4×10^6 CFU/mL). Sequencing the intestinal microbiota of zebrafish.

Bacteria_B1_raw.fq.gz、Bacteria_B2_raw.fq.gz、Bacteria_B3_raw.fq.gz :

Blank control group(B1, B2, B3 Three replicates.) ：Adult zebrafish weighing 0.2 g received no treatment. Sequencing the intestinal microbiota of zebrafish.

Bacteria_C1_raw.fq.gz、Bacteria_C2_raw.fq.gz、Bacteria_C3_raw.fq.gz :

IgY injection group（C1、C2、C3 Three replicates.）: Adult zebrafish weighing 0.2 g were injected with only 10 μL of anti-Vibrio vulnificus egg yo...,