Role of SMN Protein in regulating the transcription and alternative splicing of Pulmonary Vascular Remodeling-Related Genes in HPASMCs

To explore the effect of SMN protein on alternative splicing of multiple genes in human pulmonary artery smooth muscle cells (HPASMCs), we used shRNA specifically against the SMN to knock down the expression of SMN protein.Human primary pulmonary artery smooth muscle cells (hPASMCs) of donors were obtained from China Center for Type Culture Collection (Wuhan, Hubei, China). We then performed gene expression profiling analysis using data obtained from RNA-seq of control cells and SMN knock-down cells (each group for three biological replicates). Differentially expressed genes analysis and alternative splicing analysis of control cells and SMN knock-down cells.