HDAC Inhibitor Titration of Transcription and Axolotl Tail Regeneration

10X transcriptome sequencing of control and romidepsin treated embryonic tail regenerates. To map transcripts to axolotl cell types, single-nuclei RNA-Seq was performed. Embryos were administered 2 mm distal tail amputations and either treated in Axololt Rearing Water (N = 100) or 10 uM romidepsin (N=100). At 6 HPA, 1 mm of distal tail tip tissue was collected and pooled for nuclei isolation and 10x single nuclei RNA-Seq. Nuclei isolation, library preparation, and next generation sequencing were performed by Singulomics. The resulting data were mapped to an axolotl transcript assembly as described previously (Rodgers et al., 2020), analyzed using Cell Ranger, and visualized using 10x visualization software (Loupe version 5.0). Default graph-based clustering was used to identify distinct clusters of cells and enriched genes were used to manually identify and differentiate among cell types.