Detection of A.t.-DNA in EV-recipient cells with TaqMan-based qPCR, ExoSAP treatment and sequencing.

Shown is the total number of nested PCR replicates, the number of positive samples in TaqMan-based PCR, No. of additional negative samples included for sequencing, the summarized No. of samples which underwent sequencing and No. of samples with the correct sequence (sequencing positive). In total, PCR products of 4 primary PCR appearing positive in the SYBR Green PCR plus a negative control were applied in this experiment. The nomenclature C(-)16 stands for: EV sample C without DNase treatment C(-), qPCR No. 16 carried out with 1 μg DNA per reaction.