LC-MS raw data of Aβ40 and medin co-aggregation

This repository contains raw LC–MS data files (.lcd format) generated from analyses of Aβ40, Medin, and their co-aggregation samples. These datasets correspond to the experiments presented in Figure 1 and Supplementary Figure 2 of the associated manuscript. The files are provided in Shimadzu proprietary .lcd format. LC–MS Instrumentation and Methods Liquid chromatography analysis was performed using a Shimadzu Prominence LC-30 system coupled to a Zorbax 300 Extend-C18 analytical column (2.1 × 100 mm, 3.5 µm, 300 Å) with a matching narrow-bore guard column (2.1 × 12.5 mm, 5 µm). Mass spectrometric detection was carried out online using a Shimadzu LCMS-2020 single quadrupole detector operating in electrospray ionization (ESI) mode. Chromatographic separation was conducted at 80°C with a flow rate of 0.35 mL/min, using a binary solvent system consisting of: Solvent A: 0.1% formic acid in water Solvent B: 0.07% formic acid in acetonitrile Detection was achieved using a photodiode array (PDA) detector at 214 nm. Acquisition Details Software: Shimadzu LabSolutions v5.97 SP1 File format: .lcd (Shimadzu proprietary format) Chromatogram type: MultiChrom Time range: 0–19.008 min Sampling interval: 640 ms Intensity units: mAU Experimental Context LC-MS data for the following peptides in 50 mM Tris (pH 7.4), 100 mM NaCl, and 10 µM ThT buffer 20 µM Aβ40 peptide 20 µM Medin peptide 20 µM Aβ40–Medin co-aggregation samples