Experimentally observed and computationally predicted extracellular flux distributions for <i>S</i>. <i>fumaroxidans</i> examined in this study.

Experimental measurements from fumarate-only and propionate-only conditions were used to parameterize the model. *Monoculture simulations were performed with qualitative reaction direction constraints which prevent H2 production. #Experimental stoichiometries were calculated from experimental measurements assuming no biomass growth [36,52]. $This growth rate was predicted after the model was modified to include an explicit constraint on the propionate to fumarate uptake ratio. Experimentally observed and computationally predicted extracellular flux distributions for S. fumaroxidans examined in this study.